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ZNF480 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ZNF480 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G106934-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ZNF480 (NM_144684), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
ZNF480 | Zinc finger protein 480
Origin Species
Human
SwissProt
Q8WV37

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
59.3 kda
Protein Type
Lysate
Amino Acid Sequence
1MALPQGHLTF RDVAIEFSQA EWKCLDPAQR ALYKDVMLEN YRNLVSLGIS LPDLNINSML
61EQRREPWSGE SEVKIAKNSD GRECIKGVNT GSSYALGSNA EDKPIKKQLG VSFHLHLSEL
121ELFPDERVIN GCNQVENFIN HSSSVSCLQE MSSSVKTPIF NRNDFDDSSF LPQEQKVHLR
181EKPYECNEHS KVFRVSSSLT KHQVIHTVEK PYKCNSCGKV FSRNSHLAEH CRIHTGEKPY
241KCNVCGKVFS YNSNFARHQR IHTREKPYEC NECGKVFSNN SYLARHQRIH AEEKPYKCNE
301CGKGFSHKSS LANHWRIYTG EKPYKCDECG KAFYRIALLV RHQKIHTGEK PYKCNECGKV
361FIQNSHLAQH WRIHTGEKPY KCNECGKVFN QLSNLARHRR IHTGEKPYKC NECGKAFSEY
421SGLSAHLVIH TGEKPYKCSE CGKAFRHKLS LTNHQRIHTG ERPYKCNECG KVFNRIAHLA
481RHRKIHTGEK PYKCNECGKA FSRISYLAQH WTIHMG
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

516 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.