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ZNF471 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ZNF471 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G110969-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ZNF471 (NM_020813), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
KIAA1396 | Zinc finger protein 471 | ZNF471 | ERP1 | EZFIT-related protein 1 | Z1971
Origin Species
Human
NCBI GenBank
AB037817
SwissProt
Q9BX82

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
72.8 kDa
Protein Type
Lysate
Amino Acid Sequence
1MNVEVVKVMP QDLVTFKDVA IDFSQEEWQW MNPAQKRLYR SMMLENYQSL VSLGLCISKP
61YVISLLEQGR EPWEMTSEMT RSPFSDWESI YVTQELPLKQ FMYDDACMEG ITSYGLECST
121FEENWKWEDL FEKQMGSHEM FSKKEIITHK ETITKETEFK YTKFGKCIHL ENIEESIYNH
181TSDKKSFSKN SMVIKHKKVY VGKKLFKCNE CDKTFTHSSS LTVHFRIHTG EKPYACEECG
241KAFKQRQHLA QHHRTHTGEK LFECKECRKA FKQSEHLIQH QRIHTGEKPY KCKECRKAFR
301QPAHLAQHQR IHTGEKPYEC KECGKAFSDG SSFARHQRCH TGKRPYECIE CGKAFRYNTS
361FIRHWRSYHT GEKPFNCIDC GKAFSVHIGL ILHRRIHTGE KPYKCDVCGK TFSSGSSRTV
421HQRIHTGEKP YECDICGKDF SHHASLTQHQ RVHSGEKPYE CKECGKAFRQ NVHLVSHLRI
481HTGEKPYECK ECGKAFRISS QLATHQRIHT GEKPYECIEC GNAFKQRSHL AQHQKTHTGE
541KPYECNECGK AFSQTSNLTQ HQRIHTGEKP YKCTECGKAF SDSSSCAQHQ RLHTGQRPYQ
601CFECGKAFRR KLSLICHQRS HTGEEP
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

626 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.