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ZNF460 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ZNF460 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G115186-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Zinc finger protein 460(ZNF460) (NM_006635), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Zinc finger protein 460 | ZNF460 | Zinc finger protein HZF8 | Zinc finger protein 272 | HZF8 | ZNF272
Origin Species
Human
NCBI GenBank
NM_006635
SwissProt
Q14592

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
63.5 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAAAWMAPAQ ESVTFEDVAV TFTQEEWGQL DVTQRALYVE VMLETCGLLV ALGDSTKPET
61VEPIPSHLAL PEEVSLQEQL AQGVPRYSYL GQAMDQDGPS EMQEYFLRPG TDPQSEKLHG
121KMSLEHEGLA TADGICSMMI QNQVSPEDAL YGFDSYGPVT DSLIHEGENS YKFEEMFNEN
181CFLVQHEQIL PRVKPYDCPE CGKAFGKSKH LLQHHIIHTG EKPYKCLECG KDFNRRSHLT
241RHQRTHNGDK PFVCSECGRT FNRGSHLTRH QRVHSGEKPF VCNECGKAFT YRSNFVLHNK
301SHNEKKPFAC SECGKGFYES TALIQHFIIH TGERPFKCLE CGKAFNCRSH LKQHERIHTG
361EKPFVCSQCG KAFTHYSTYV LHERAHTGEK PFECKECGKA FSIRKDLIRH FNIHTGEKPY
421ECLQCGKAFT RMSGLTRHQW IHTGEKPYVC IQCGKAFCRT TNLIRHFSIH TGEKPYECVE
481CGKAFNRRSP LTRHQRIHTA EKSHEPIQSG NVSCESTDLI QHSIIHTESS PVSAVNMETP
541SIAAHSSSLD INGFIVEETL PL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

562 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.