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ZNF45 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ZNF45 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G100046-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ZNF45 (NM_003425), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
BRC1744 | Zinc finger protein 45 | Zinc finger protein KOX5 | ZNF45 | KOX5 | Zinc finger protein 13 | ZNF13
Origin Species
Human
NCBI GenBank
NM_003425
SwissProt
Q02386

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
78.1 kDa
Protein Type
Lysate
Amino Acid Sequence
1MTKSKEAVTF KDVAVVFSEE ELQLLDLAQR KLYRDVMLEN FRNVVSVGHQ STPDGLPQLE
61REEKLWMMKM ATQRDNSSGA KNLKEMETLQ EVGLRYLPHE ELFCSQIWQQ ITRELIKYQD
121SVVNIQRTGC QLEKRDDLHY KDEGFSNQSS HLQVHRVHTG EKPYKGEHCV KSFSWSSHLQ
181INQRAHAGEK PYKCEKCDNA FRRFSSLQAH QRVHSRAKSY TNDASYRSFS QRSHLPHHQR
241VPTGENPYKY EECGRNVGKS SHCQAPLIVH TGEKPYKCEE CGVGFSQRSY LQVHLKVHTG
301KKPYKCEECG KSFSWRSRLQ AHERIHTGEK PYKCNACGKS FSYSSHLNIH CRIHTGEKPY
361KCEECGKGFS VGSHLQAHQI SHTGEKPYKC EECGKGFCRA SNLLDHQRGH TGEKPYQCDA
421CGKGFSRSSD FNIHFRVHTG EKPYKCEECG KGFSQASNLL AHQRGHTGEK PYKCGTCGKG
481FSRSSDLNVH CRIHTGEKPY KCERCGKAFS QFSSLQVHQR VHTGEKPYQC AECGKGFSVG
541SQLQAHQRCH TGEKPYQCEE CGKGFCRASN FLAHRGVHTG EKPYRCDVCG KRFRQRSYLQ
601AHQRVHTGER PYKCEECGKV FSWSSYLQAH QRVHTGEKPY KCEECGKGFS WSSSLIIHQR
661VHADDEGDKD FPSSEDSHRK TR
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

682 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.