LSBio
ZNF449 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ZNF449 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G102371-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ZNF449 (NM_152695), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
ZSCAN19 | Zinc finger protein 449 | ZNF449
Origin Species
Human
SwissProt
Q6P9G9

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
59.8 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAVALGCAIQ ASLNQGSVFQ EYDTDCEVFR QRFRQFQYRE AAGPHEAFNK LWELCCQWLK
61PKMRSKEQIL ELLVLEQFLT ILPTEIETWV REHCPENRER VVSLIEDLQR ELEIPEQQVD
121MHDMLLEELA PVGTAHIPPT MHLESPALQV MGPAQEAPVA EAWIPQAGPP ELNYGATGEC
181QNFLDPGYPL PKLDMNFSLE NREEPWVKEL QDSKEMKQLL DSKIGFEIGI ENEEDTSKQK
241KMETMYPFIV TLEGNALQGP ILQKDYVQLE NQWETPPEDL QTDLAKLVDQ QNPTLGETPE
301NSNLEEPLNP KPHKKKSPGE KPHRCPQCGK CFARKSQLTG HQRIHSGEEP HKCPECGKRF
361LRSSDLYRHQ RLHTGERPYE CTVCKKRFTR RSHLIGHQRT HSEEETYKCL ECGKSFCHGS
421SLKRHLKTHT GEKPHRCHNC GKSFSRLTAL TLHQRTHTEE RPFKCNYCGK SFRQRPSLVI
481HLRIHTGEKP YKCTHCSKSF RQRAGLIMHQ VTHFRGLI
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

518 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.