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ZNF436 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ZNF436 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G119949-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ZNF436 (NM_001077195), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
DNA-binding protein | ZNF | ZNF436 | KIAA1710 | Zfp46 | Zinc finger protein 436
Origin Species
Human
NCBI GenBank
NM_001077195
SwissProt
Q9C0F3

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
54.1 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAATLLMAGS QAPVTFEDMA MYLTREEWRP LDAAQRDLYR DVMQENYGNV VSLDFEIRSE
61NEVNPKQEIS EDVQFGTTSE RPAENAEENP ESEEGFESGD RSERQWGDLT AEEWVSYPLQ
121PVTDLLVHKE VHTGIRYHIC SHCGKAFSQI SDLNRHQKTH TGDRPYKCYE CGKGFSRSSH
181LIQHQRTHTG ERPYDCNECG KSFGRSSHLI QHQTIHTGEK PHKCNECGKS FCRLSHLIQH
241QRTHSGEKPY ECEECGKSFS RSSHLAQHQR THTGEKPYEC NECGRGFSER SDLIKHYRVH
301TGERPYKCDE CGKNFSQNSD LVRHRRAHTG EKPYHCNECG ENFSRISHLV QHQRTHTGEK
361PYECNACGKS FSRSSHLITH QKIHTGEKPY ECNECWRSFG ERSDLIKHQR THTGEKPYEC
421VQCGKGFTQS SNLITHQRVH TGEKPYECTE CEKSFSRSSA LIKHKRVHTD
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

470 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.