Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
ZNF435 / ZSCAN16 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G109468-100
Catalog Number: LS-G109468
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ZSCAN16 (NM_025231), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- DJ265C24.3 | ZNF392 | ZSCAN16 | Zinc finger protein 392 | Zinc finger protein 435 | ZNF435
- Origin Species
- Human
- NCBI GenBank
- NM_025231
- SwissProt
- Q9H4T2
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 40.6 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MTTALEPEDQ KGLLIIKAED HYWGQDSSSQ KCSPHRRELY RQHFRKLCYQ DAPGPREALT61QLWELCRQWL RPECHTKEQI LDLLVLEQFL SILPKDLQAW VRAHHPETGE EAVTVLEDLE121RELDEPGKQV PGNSERRDIL MDKLAPLGRP YESLTVQLHP KKTQLEQEAG KPQRNGDKTR181TKNEELFQKE DMPKDKEFLG EINDRLNKDT PQHPKSKDII ENEGRSEWQQ RERRRYKCDE241CGKSFSHSSD LSKHRRTHTG EKPYKCDECG KAFIQRSHLI GHHRVHTGVK PYKCKECGKD301FSGRTGLIQH QRIHTGEKPY ECDECGRPFR VSSALIRHQR IHTANKLYTR TRPLEQKLIS361EEDLAANDIL DYKDDDDKV
379 aa
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.