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ZNF433 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ZNF433 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G120198-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ZNF433 (NM_001080411), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
ZNF433 | Zinc finger protein 433
Origin Species
Human
SwissProt
Q8N7K0

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
77.1 kDa
Protein Type
Lysate
Amino Acid Sequence
1MMFQDSVAFE DVAVTFTQEE WALLDPSQKN LCRDVMQETF RNLASIGKKW KPQNIYVEYE
61NLRRNLRIVG ERLFESKEGH QHGEILTQVP DDMLKKTTTG VKSCESSVYG EVGSAHSSLN
121RHIRDDTGHK AYEYQEYGQK PYKCKYCKKP FNCLSSVQTH ERAHSGRKLY VCEECGKTFI
181SHSNLQRHRI MHRGDGPYKC KFCGKALMFL SLYLIHKRTH TGEKPYQCKQ CGKAFSHSSS
241LRIHERTHTG EKPYKCNECG KAFHSSTCLH AHKRTHTGEK PYECKQCGKA FSSSHSFQIH
301ERTHTGEKPY ECKECGKAFK CPSSVRRHER THSRKKPYEC KHCGKVLSYL TSFQNHLGMH
361TGEISHKCKI CGKAFYSPSS LQTHEKTHTG EKPYKCNQCG KAFNSSSSFR YHERTHTGEK
421PYECKQCGKA FRSASLLQTH GRTHTGEKPY ACKECGKPFS NFSFFQIHER MHREEKPYEC
481KGYGKTFSLP SLFHRHERTH TGGKTYECKQ CGRSFNCSSS FRYHGRTHTG EKPYECKQCG
541KAFRSASQLQ IHGRTHTGEK PYECKQCGKA FGSASHLQMH GRTHTGEKPY ECKQCGKSFG
601CASRLQMHGR THTGEKPYKC KQCGKAFGCP SNLRRHGRTH TGEKPYKCNQ CGKVFRCSSQ
661LQVHGRAHCI DTP
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

673 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.