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ZNF426 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ZNF426 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all ZNF426 antibodies →
$504.00each
Catalog Number: LS-G110029-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ZNF426 (NM_024106), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Zinc finger protein 426 | ZNF426
Origin Species
Human
NCBI GenBank
NM_024106
SwissProt
Q9BUY5

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
62.9 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAAADLSHGH YLSGDPVCLH EEKTPAGRIV ADCLTDCYQD SVTFDDVAVD FTQEEWTLLD
61STQRSLYSDV MLENYKNLAT VGGQIIKPSL ISWLEQEESR TVQGGVLQGW EMRLETQWSI
121LQQDFLRGQT SIGIQLEGKH NGRELCDCEQ CGEVFSEHSC LKTHVRTQST GNTHDCNQYG
181KDFLTLCEKT STGEKLSEFN QSEKIFSLTP NIVYQRTSTQ EKSFECSHCG KSFINESYLQ
241AHMRTHNGEK LYEWRNYGPG FIDSTSLSVL IETLNAKKPY KCKECGKGYR YPAYLSIHMR
301THTGEKPYEC KECGKAFNYS NSFQIHGRTH TGEKPYVCKE CGKAFTQYSG LSMHVRSHSG
361DKPYECKECG KSFLTSSRLI QHIRTHTGEK PFVCVECGKA FAVSSNLSGH LRTHTEEKAC
421ECKICGKVFG YPSCLNNHMR THSAQKPYTC KECGKAFNYS THLKIHMRIH TGEKPYECKQ
481CGKAFSHSSS FQIHERTHTG EKPYECKECG KAFTCSSSFR IHEKTHTEEK PYKCQQCGKA
541YSHPRSLRRH EQIH
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

554 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.