LSBio
ZNF420 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ZNF420 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all ZNF420 antibodies →
$504.00each
Catalog Number: LS-G106896-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ZNF420 (NM_144689), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
APAK | Zinc finger protein 420 | ZNF420
Origin Species
Human
SwissProt
Q8TAQ5

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
80.1 kDa
Protein Type
Lysate
Amino Acid Sequence
1MARKLVMFRD VAIDFSQEEW ECLDSAQRDL YRDVMLENYS NLVSLDLPSR CASKDLSPEK
61NTYETELSQW EMSDRLENCD LEESNSRDYL EAKGKMEKQQ ENQKEYFRQG MIIYDKMSIF
121NQHTYLSQHS RCHSTEKPYK CKECGKAFRR ASHLTQHQSI HTGEKPYECK QCGKAFSRDS
181QLSLHQRLHT GEKPYACKEC GKAFTQSSQL ILHHRIHTGE KPYKCEECGK AFIRSSQLTR
241HQKVHTGEKP YECKECGKAF TQNSQLTLHQ RLHTGEKLYE CKECRKVFTQ LSQLILHKRI
301HTGEKPYECK ECGKAFICGS QLSQHQKIHN GEKPYECKEC GRAFIRGSLL MQHQRIHTGE
361KPYKCEECGK AFIRGSQLTQ HQRIHTNEKP YECKECGKMF SHGSQLTQHQ RIHTGEKPYQ
421CKECGKAFNR GSLLTRHQRI HTGEKPYECK ECGKTFSRGS ELTQHERIHT GEKPYECKEC
481GKSFIRGSQL TQHQRIHTGE KPYECKECRM AFTQSSHLSQ HQRLHTGEKP YVCNECGKAF
541ARGLLLIQHQ RIHTGEKPYQ CKECGKAFIR GSQLTQHQRI HTGEKPYECK ECGKAFSHGS
601QLTLHQRIHT GEKPYECREC RKAFTQSSHL SRHQRIHTGE KPYQCKECGK AFTRGSQLTQ
661HQRIHISEKS FEYKECGIDF SHGSQVYM
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

688 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.