LSBio
ZNF418 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ZNF418 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all ZNF418 antibodies →
$725.00each
Catalog Number: LS-G107506-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ZNF418 (NM_133460), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
KIAA1956 | Zinc finger protein 418 | ZNF418
Origin Species
Human
SwissProt
Q8TF45

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
77.7 kda
Protein Type
Lysate
Amino Acid Sequence
1MQGTVAFEDV AVNFSQEEWS LLSEVQRCLY HDVMLENWVL ISSLGCWCGS EDEEAPSKKS
61ISIQRVSQVS TPGAGVSPKK AHSCEMCGAI LGDILHLADH QGTHHKQKLH RCEAWGNKLY
121DSSNRPHQNQ YLGEKPYRSS VEEALFVKRC KFHVSEESSI FIQSGKDFLP SSGLLLQEAT
181HTGEKSNSKP ECESPFQWGD THYSCGECMK HSSTKHVFVQ QQRLPSREEC YCWECGKSFS
241KYDSVSNHQR VHTGKRPYEC GECGKSFSHK GSLVQHQRVH TGKRPYECGE CGKSFSHKGS
301LVQHQRVHTG ERPYECGECG KSFSQNGTLI KHQRVHTGER PYECEECGKC FTQKGNLIQH
361QRGHTSERPY ECEECGKCFS QKGTLTEHHR VHTRERPYEC GECGKSFSRK GHLRNHQRGH
421TGERPYECGE CGKSFSRKGN LIQHQRSHTG ERPYECRECR KLFRGKSHLI EHQRVHTGER
481PYECNECGKS FQDSSGFRVH QRVHTGEKPF ECSECGKSFP QSCSLLRHRR VHTGERPYEC
541GECGKSFHQS SSLLRHQKTH TAERPYECRE CGKFFSSLLE HRRVHTGERP YECRECGKTF
601TRRSAHFKHQ RLHTRGKPYE CSECGKSFAE TFSLTEHRRV HTGERPYECS ECGKSFHRSS
661SLLRHQRVHT ERSPYK
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

676 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.