Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
ZNF408 / PRDM17 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G101905-100
Catalog Number: LS-G101905
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ZNF408 (NM_024741), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- PFM14 | Zinc finger protein 408 | ZNF408 | PRDM17
- Origin Species
- Human
- NCBI GenBank
- NM_024741
- SwissProt
- Q9H9D4
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 78.3 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MEEAEELLLE GKKALQLARE PRLGLDLGWN PSGEGCTQGL KDVPPEPTRD ILALKSLPRG61LALGPSLAKE QRLGVWCVGD PLQPGLLWGP LEEESASKEK GEGVKPRQEE NLSLGPWGDV121CACEQSSGWT SLVQRGRLES EGNVAPVRIS ERLHLQVYQL VLPGSELLLW PQPSSEGPSL181TQPGLDKEAA VAVVTEVESA VQQEVASPGE DAAEPCIDPG SQSPSGIQAE NMVSPGLKFP241TQDRISKDSQ PLGPLLQDGD VDEECPAQAQ MPPELQSNSA TQQDPDGSGA SFSSSARGTQ301PHGYLAKKLH SPSDQCPPRA KTPEPGAQQS GFPTLSRSPP GPAGSSPKQG RRYRCGECGK361AFLQLCHLKK HAFVHTGHKP FLCTECGKSY SSEESFKAHM LGHRGVRPFP CPQCDKAYGT421QRDLKEHQVV HSGARPFACD QCGKAFARRP SLRLHRKTHQ VPAAPAPCPC PVCGRPLANQ481GSLRNHMRLH TGEKPFLCPH CGRAFRQRGN LRGHLRLHTG ERPYRCPHCA DAFPQLPELR541RHLISHTGEA HLCPVCGKAL RDPHTLRAHE RLHSGERPFP CPQCGRAYTL ATKLRRHLKS601HLEDKPYRCP TCGMGYTLPQ SLRRHQLSHR PEAPCSPPSV PSAASEPTVV LLQAEPQLLD661THREEEVSPA RDVVEVTISE SQEKCFVVPE EPDAAPSLVL IHKDMGLGAW AEVVEVEMGTTRTRPLEQKLISEEDLAANDILDYKDDDDKV
720 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.