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ZNF394 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ZNF394 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G102031-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ZNF394 (NM_032164), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
ZNF394 | Zinc finger protein 394 | Zinc finger protein 99 | ZSCAN46
Origin Species
Human
NCBI GenBank
NM_032164
SwissProt
Q53GI3

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
64.1 kDa
Protein Type
Lysate
Amino Acid Sequence
1MNSSLTAQRR GSDAELGPWV MAARSKDAAP SQRDGLLPVK VEEDSPGSWE PNYPAASPDP
61ETSRLHFRQL RYQEVAGPEE ALSRLRELCR RWLRPELLSK EQILELLVLE QFLTILPEEL
121QAWVREHCPE SGEEAVAVVR ALQRALDGTS SQGMVTFEDT AVSLTWEEWE RLDPARRDFC
181RESAQKDSGS TVPPSLESRV ENKELIPMQQ ILEEAEPQGQ LQEAFQGKRP LFSKCGSTHE
241DRVEKQSGDP LPLKLENSPE AEGLNSISDV NKNGSIEGED SKNNELQNSA RCSNLVLCQH
301IPKAERPTDS EEHGNKCKQS FHMVTWHVLK PHKSDSGDSF HHSSLFETQR QLHEERPYKC
361GNCGKSFKQR SDLFRHQRIH TGEKPYGCQE CGKSFSQSAA LTKHQRTHTG EKPYTCLKCG
421ERFRQNSHLN RHQSTHSRDK HFKCEECGET CHISNLFRHQ RLHKGERPYK CEECEKSFKQ
481RSDLFKHHRI HTGEKPYGCS VCGKRFNQSA TLIKHQRIHT GEKPYKCLEC GERFRQSTHL
541IRHQRIHQNK VLSAGRGGSR L
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

561 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.