Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
ZNF385B / ZNF533 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G106201-100
Catalog Number: LS-G106201
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ZNF385B (NM_152520), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Zinc finger protein 385B | Zinc finger protein 533 | ZNF533 | ZNF385B | ZFN533
- Origin Species
- Human
- NCBI GenBank
- AA460775
- SwissProt
- Q569K4
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 50.2 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MNMANFLRGF EEKGIKNDRP EDQLSKEKKK ILFSFCEVCN IQLNSAAQAQ VHSNGKSHRK61RVKQLSDGQP PPPAQASPSS NSSTGSTCHT TTLPALVRTP TLMMQPSLDI KPFMSFPVDS121SSAVGLFPNF NTMDPVQKAV INHTFGVSIP PKKKQVISCN VCQLRFNSDS QAEAHYKGSK181HAKKVKALDA TKNKPKMVPS KDSAKANPSC SITPITGNNS DKSEDKGKLK ASSSSQPSSS241ESGSFLLKSG TTPLPPGAAT SPSKSTNGAP GTVVESEEEK AKKLLYCSLC KVAVNSLSQL301EAHNTGSKHK TMVEARNGAG PIKSYPRPGS RLKMQNGSKG SGLQNKTFHC EICDVHVNSE361IQLKQHISSR RHKDRVAGKP LKPKYSPYNK LQRSPSILAA KLAFQKDMMK PLAPAFLSSP421LAAAAAVSSA LSLPPRPSAS LFQAPAIPPA LLRPGHGPIR ATPASILFAP YTRTRPLEQKLISEEDLAANDILDYKDDDDKV
471 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.