Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
ZNF385A / ZNF385 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G113186-100
Catalog Number: LS-G113186
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ZNF385(ZNF385A) (NM_015481), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- HZF | ZNF385A | Zinc finger protein 385 | Zinc finger protein 385A | ZFP385 | ZNF385 | Retinal zinc finger protein | RZF
- Origin Species
- Human
- NCBI GenBank
- NM_015481
- SwissProt
- Q96PM9
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 38.2 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MQPPLDLKQI LPFPLEPAPT LGLFSNYSTM DPVQKAVLSH TFGGPLLKTK RPVISCNICQ61IRFNSQSQAE AHYKGNRHAR RVKGIEAAKT RGREPGVREP GDPAPPGSTP TNGDGVAPRP121VSMENGLGPA PGSPEKQPGS PSPPSIPETG QGVTKGEGGT PAPASLPGGS KEEEEKAKRL181LYCALCKVAV NSLSQLEAHN KGTKHKTILE ARSGLGPIKA YPRLGPPTPG EPEAPAQDRT241FHCEICNVKV NSEVQLKQHI SSRRHRDGVA GKPNPLLSRH KKSRGAGELA GTLTFSKELP301KSLAGGLLPS PLAVAAVMAA AAGSPLSLRP APAAPLLQGP PITHPLLHPA PGPIRTAHGP361ILFSPYTRTRPLEQKLISEEDLAANDILDYKDDDDKV
366 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.