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ZNF384 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ZNF384 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G120412-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ZNF384 (NM_001039916), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
CIZ | CAG repeat protein 1 | CAGH1 | ERDA2 | NP | Nuclear matrix protein 4 | Zinc finger protein 384 | ZNF384 | CAGH1A | NMP4 | TNRC1
Origin Species
Human
NCBI GenBank
U80760
SwissProt
Q8TF68

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
55.9 kDa
Protein Type
Lysate
Amino Acid Sequence
1MEESHFNSNP YFWPSIPTVS GQIENTMFIN KMKDQLLPEK GCGLAPPHYP TLLTVPASVS
61LPSGISMDTE SKSDQLTPHS QASVTQNITV VPVPSTGLMT AGVSCSQRWR REGSQSRGPG
121LVITSPSGSL VTTASSAQTF PISAPMIVSA LPPGSQALQV VPDLSKKVAS TLTEEGGGGG
181GGGGSVAPKP PRGRKKKRML ESGLPEMNDP YVLSPEDDDD HQKDGKTYRC RMCSLTFYSK
241SEMQIHSKSH TETKPHKCPH CSKTFANSSY LAQHIRIHSG AKPYSCNFCE KSFRQLSHLQ
301QHTRIHTGDR PYKCAHPGCE KAFTQLSNLQ SHRRQHNKDK PFKCHNCHRA YTDAASLEVH
361LSTHTVKHAK VYTCTICSRA YTSETYLMKH MRKHNPPDLQ QQVQAAAAAA AVAQAQAQAQ
421AQAQAQAQAQ AQAQASQASQ QQQQQQQQQQ QQQQQPPPHF QSPGAAPQGG GGGDSNPNPP
481PQCSFDLTPY KTAEHHKDIC LTVTTSTIQV EHLASS
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

516 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.