LSBio
ZNF366 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ZNF366 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all ZNF366 antibodies →
$725.00each
Catalog Number: LS-G106155-100

For bulk orders or custom formulations:

Request a Quote

Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ZNF366 (NM_152625), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
DCSCRIPT | Zinc finger protein 366 | ZNF366
Origin Species
Human
SwissProt
Q8N895

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
84.9 kda
Protein Type
Lysate
Amino Acid Sequence
1MQKEMKMIKD EDVHFDLAVK KTPSFPHCLQ PVASRGKAPQ RHPFPEALRG PFSQFRYEPP
61PGDLDGFPGV FEGAGSRKRK SMPTKMPYNH PAEEVTLALH SEENKNHGLP NLPLLFPQPP
121RPKYDSQMID LCNVGFQFYR SLEHFGGKPV KQEPIKPSAV WPQPTPTPFL PTPYPYYPKV
181HPGLMFPFFV PSSSPFPFSR HTFLPKQPPE PLLPRKAEPQ ESEETKQKVE RVDVNVQIDD
241SYYVDVGGSQ KRWQCPTCEK SYTSKYNLVT HILGHSGIKP HACTHCGKLF KQLSHLHTHM
301LTHQGTRPHK CQVCHKAFTQ TSHLKRHMMQ HSEVKPHNCR VCGRGFAYPS ELKAHEAKHA
361SGRENICVEC GLDFPTLAQL KRHLTTHRGP IQYNCSECDK TFQYPSQLQN HMMKHKDIRP
421YICSECGMEF VQPHHLKQHS LTHKGVKEHK CGICGREFTL LANMKRHVLI HTNIRAYQCH
481LCYKSFVQKQ TLKAHMIVHS DVKPFKCKLC GKEFNRMHNL MGHMHLHSDS KPFKCLYCPS
541KFTLKGNLTR HMKVKHGVME RGLHSQGLGR GRIALAQTAG VLRSLEQEEP FDLSQKRRAK
601VPVFQSDGES AQGSHCHEEE EEDNCYEVEP YSPGLAPQSQ QLCTPEDLST KSEHAPEVLE
661EACKEEKEDA SKGEWEKRSK GDLGAEGGQE RDCAGRDECL SLRAFQSTRR GPSFSDYLYF
721KHRDESLKEL LERKMEKQAV LLGI
SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV

744 aa + 34 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.