LSBio
ZNF362 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ZNF362 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all ZNF362 antibodies →
$504.00each
Catalog Number: LS-G106225-100

For bulk orders or custom formulations:

Request a Quote

Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ZNF362 (NM_152493), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Lin-29 | RN | Rotund homolog | Zinc finger protein 362 | ZNF362
Origin Species
Human
NCBI GenBank
NM_152493
SwissProt
Q5T0B9

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
45.6 kda
Protein Type
Lysate
Amino Acid Sequence
1MSRSSPSGKG HSRMAEPRFN NPYFWPPPPT MPSQLDNLVL INKIKEQLMA EKIRPPHLPP
61TSASSQQPLL VPPAPAESSQ AVMSLPKLQQ VPGLHPQAVP QPDVALHARP ATSTVTGLGL
121STRTPSVSTS ESSAGAGTGT GTSTPSTPTT TSQSRLIASS PTLISGITSP PLLDSIKTIQ
181GHGLLGPPKS ERGRKKIKAE NPGGPPVLVV PYPILASGET AKEGKTYRCK VCPLTFFTKS
241EMQIHSKSHT EAKPHKCPHC SKSFANASYL AQHLRIHLGV KPYHCSYCDK SFRQLSHLQQ
301HTRIHTGDRP YKCPHPGCEK AFTQLSNLQS HQRQHNKDKP YKCPNCYRAY SDSASLQIHL
361SAHAIKHAKA YCCSMCGRAY TSETYLMKHM SKHTVVEHLV SHHSPQRTES PGIPVRISLI
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

420 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.