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ZNF354C Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ZNF354C Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G113842-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ZNF354C (NM_014594), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
HKID3 | KID3 | Zinc finger protein 354C | ZNF354C
Origin Species
Human
SwissProt
Q86Y25

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
56.6 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAVDLLSAQE PVTFRDVAVF FSQDEWLHLD SAQRALYREV MLENYSSLVS LGIPFSMPKL
61IHQLQQGEDP CMVEREVPSD TRLGFKTWLE TEALPHRQDI FIEETSQGMV KKESIKDGHW
121DINFEEAVEF ESEIEEEQEK KPLRQMIDSH EKTISEDGNH TSLELGKSLF TNTALVTQQS
181VPIERIPNMY YTFGKDFKQN FDLMKCFQIY PGGKPHICNE CGKSFKQNLH LIEHQRIHTG
241EKPYKCNECK KTFSHRSSLL SHQRIHTGEK PYKCNECEKA FSNSSTLIKH LRVHTGEKPY
301RCRECGKAFS QCSTLTVHQR IHTGEKLYKC GECEKAFNCR AKLHRHQRIH TGEKPYKCSE
361CGKGYSQFTS LAEHQRFHTG EQLYTCLECG RTFTRIVTLI EHQRIHTGQK PYQCNECEKA
421FNQYSSFNEH RKIHTGEKLY TCEECGKAFG CKSNLYRHQR IHTGEKPYQC NQWKGLQPVF
481IFNRT
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

485 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.