LSBio
ZNF354B Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ZNF354B Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all ZNF354B antibodies →
$504.00each
Catalog Number: LS-G107944-100

For bulk orders or custom formulations:

Request a Quote

Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ZNF354B (NM_058230), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
KID2 | Zinc finger protein 354B | ZNF354B
Origin Species
Human
NCBI GenBank
NM_058230
SwissProt
Q96LW1

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
70.3 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAAGQREARP QVSLTFEDVA VLFTWDEWRK LAPSQRNLYR DVMLENYRNL VSLGLSFTKP
61KVISLLQQGE DPWEVEKDSS GVSSLGCKST PKMTKSTQTQ DSFQEQIRKR LKRDEPWNFI
121SERSCIYEEK LKKQQDKNEN LQIISVAHTK ILTVDRSHKN VEFGQNFYLK SVFIKQQRFA
181KEKTPSKCEI QRNSFKQNSN LLNQSKIKTA EKRYKCSTCE KAFIHNSSLR KHQKNHTGEK
241LFKCKECLKA FSQSSALIQH QRTHTGEKPY ICKECGKAFS HSASLCKHLR THTVEKCYRC
301KECGKSFSRR SGLFIHQKIH AQENPHKYNP GRKASSYSTS LSGSQKIHLR KKSYLCNECG
361NTFKSSSSLR YHQRIHTGEK PFKCSECGRA FSQSASLIQH ERIHTGEKPY RCNECGKGFT
421SISRLNRHRI IHTGEKLYNC NECGKALSSH STLIIHERIH TGEKPCKCKV CGKAFRQSSA
481LIQHQRMHTG ERPYKCNECD KTFRCNSSLS NHQRIHTGEK PYRCLECGMS FGQSAALIQH
541QRIHTGEKPF KCNTCGKTFR QSSSLIAHQR IHTGEKPYEC NACGKLFSQR SSLTNHYKIH
601IEEDSLKADL HV
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

612 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.