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ZNF354A Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ZNF354A Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G115791-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ZNF354A (NM_005649), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
HKL1 | EZNF | KID-1 | KID1 | TCF-17 | TCF17 | Zinc finger protein 354A | Zinc finger protein eZNF | ZNF354A | Transcription factor 17
Origin Species
Human
NCBI GenBank
NM_005649
SwissProt
O60765

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
69.1 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAAGQREARP QVSLTFEDVA VLFTRDEWRK LAPSQRNLYR DVMLENYRNL VSLGLPFTKP
61KVISLLQQGE DPWEVEKDGS GVSSLGSKSS HKTTKSTQTQ DSSFQGLILK RSNRNVPWDL
121KLEKPYIYEG RLEKKQDKKG SFQIVSATHK KIPTIERSHK NTELSQNFSP KSVLIRQQIL
181PREKTPPKCE IQGNSLKQNS QLLNQPKITA DKRYKCSLCE KTFINTSSLR KHEKNHSGEK
241LFKCKECSKA FSQSSALIQH QITHTGEKPY ICKECGKAFT LSTSLYKHLR THTVEKSYSC
301KECGKSFSRR SGLFIHQKIH AEENPCKYNP GRKASSCSTS LSGCQRIHSR KKSYLCNECG
361NTFKSSSSLR YHQRIHTGEK PFKCSECGRA FSQSASLIQH ERIHTGEKPY RCNECGKGFT
421SISRLNRHRI IHTGEKFYNC NECGKALSSH STLIIHERIH TGEKPCKCKV CGKAFRQSSA
481LIQHQRMHTG ERPYKCNECG KTFRCNSSLS NHQRIHTGEK PYRCEECGIS FGQSSALIQH
541RRIHTGEKPF KCNTCGKTFR QSSSRIAHQR IHTGEKPYEC NTCGKLFNHR SSLTNHYKIH
601IEEDP
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

605 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.