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ZNF350 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ZNF350 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G110646-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ZNF350 (NM_021632), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
KRAB zinc finger protein ZFQR | ZBRK1 | ZFQR | ZNF350 | Zinc finger protein ZBRK1 | Zinc finger protein 350 | Zinc-finger protein ZBRK1
Origin Species
Human
NCBI GenBank
NM_021632
SwissProt
Q9GZX5

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
59.8 kDa
Protein Type
Lysate
Amino Acid Sequence
1MIQAQESITL EDVAVDFTWE EWQLLGAAQK DLYRDVMLEN YSNLVAVGYQ ASKPDALFKL
61EQGEQLWTIE DGIHSGACSD IWKVDHVLER LQSESLVNRR KPCHEHDAFE NIVHCSKSQF
121LLGQNHDIFD LRGKSLKSNL TLVNQSKGYE IKNSVEFTGN GDSFLHANHE RLHTAIKFPA
181SQKLISTKSQ FISPKHQKTR KLEKHHVCSE CGKAFIKKSW LTDHQVMHTG EKPHRCSLCE
241KAFSRKFMLT EHQRTHTGEK PYECPECGKA FLKKSRLNIH QKTHTGEKPY ICSECGKGFI
301QKGNLIVHQR IHTGEKPYIC NECGKGFIQK TCLIAHQRFH TGKTPFVCSE CGKSCSQKSG
361LIKHQRIHTG EKPFECSECG KAFSTKQKLI VHQRTHTGER PYGCNECGKA FAYMSCLVKH
421KRIHTREKQE AAKVENPPAE RHSSLHTSDV MQEKNSANGA TTQVPSVAPQ TSLNISGLLA
481NRNVVLVGQP VVRCAASGDN RGFAQDRNLV NAVNVVVPSV INYVLFYVTE NP
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

532 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.