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ZNF34 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ZNF34 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G109475-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ZNF34 (NM_030580), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
KOX32 | Zinc finger protein 34 | Zinc finger protein KOX32 | ZNF34
Origin Species
Human
NCBI GenBank
NM_030580
SwissProt
Q8IZ26

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
63.9 kDa
Protein Type
Lysate
Amino Acid Sequence
1MMVGVALRPP CSHPLGCISW LGGRWVRPPT VLLPLACFCC LPAPTSPLWT TPGLQQSLSF
61QAEVTFEDVA VYLSREEWGR LGPAQRGLYR DVMLETYGNL VSLALGTRTE YKELTSQETF
121GEEDPQGSEP VEACDHISKS EGSLEKLVEQ RGPRAVTLTN GESSRESGGN LRLLSRPVPD
181QRPHKCDICE QSFEQRSYLN NHKRVHRSKK TNTVRNSGEI FSANLVVKED QKIPTGKKLH
241YCSYCGKTFR YSANLVKHQR LHTEEKPYKC DECGKAFSQS CEFINHRRMH SGEIPYRCDE
301CGKTFTRRPN LMKHQRIHTG EKPYKCGECG KHFSAYSSLI YHQRIHTGEK PYKCNDCGKA
361FSDGSILIRH RRTHTGEKPF ECKECGKGFT QSSNLIQHQR IHTGEKPYKC NECEKAFIQK
421TKLVEHQRSH TGEKPYECND CGKVFSQSTH LIQHQRIHTG EKPYKCSECG KAFHNSSRLI
481HHQRLHHGEK PYRCSDCKKA FSQSTYLIQH RRIHTGEKPY KCSECGKAFR HSSNMCQHQR
541IHLREDFSM
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

549 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.