LSBio
ZNF334 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ZNF334 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all ZNF334 antibodies →
$504.00each
Catalog Number: LS-G111906-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ZNF334 (NM_018102), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
BA179N14.1 | Zinc finger protein 334 | ZNF334
Origin Species
Human
NCBI GenBank
NM_018102
SwissProt
Q9HCZ1

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
79.5 kDa
Protein Type
Lysate
Amino Acid Sequence
1MKMKKFQIPV SFQDLTVNFT QEEWQQLDPA QRLLYRDVML ENYSNLVSVG YHVSKPDVIF
61KLEQGEEPWI VEEFSNQNYP DIDDALEKNK EIQDKHLTQT VFFSNKTLIT ERENVFGKTL
121NLGMNSVPSR KMPYKCNPGG NSLKTNSEVI VAKKSKENRK IPDGYSGFGK HEKSHLGMKK
181YRYNPMRKAS NQNENLILHQ NIQILKQPFD YNKCGKTFFK RAILITQKGR QTERKPNECN
241ECRKTFSKRS TLIVHQRIHT GEKPYVCSDC RKTFRVKTSL TRHRRIHTGE RPYECSECRK
301TFIDKSALIV HQKIHGGEKS YECNECGKTF FRKSALAEHF RSHTGEKPYE CKECGNAFSK
361KSYLVVHQRT HRGEKPNECK ECGKTFFCQS ALTAHQRIHT GEKPYECSEC EKTFFCQSAL
421NVHRRSHTGE KPYECSQCGK FLCTKSALIA HQITHRGKKS YECNECGKFF CHKSTLTIHQ
481RTHTGEKHGV FNKCGRISIV KSNCSQCKRM NTKENLYECS EHGHAVSKNS HLIVHQRTIW
541ERPYECNECG RTYCRKSALT HHQRTHTGQR PYECNECGKT FCQKFSFVEH QRTHTGEKPY
601ECNECGKSFC HKSAFRVHRR IHTGEKPYEC NQCGKTYRRL WTLTEHQKIH TGEKPYECNK
661CEKTFRHKSN FLLHQKSHKE
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

680 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.