LSBio
ZNF333 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ZNF333 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G108820-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ZNF333 (NM_032433), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
KIAA1806 | ZNF333 | Zinc finger protein 333
Origin Species
Human
NCBI GenBank
AA975466

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
75.4 kDa
Protein Type
Lysate
Amino Acid Sequence
1MESVTFEDVA VEFIQEWALL DSARRSLCKY RMLDQCRTLA SRGTPPCKPS CVSQLGQRAE
61PKATERGILR ATGVAWESQL KPEELPSMQD LLEEASSRDM QMGPGLFLRM QLVPSIEERE
121TPLTREDRPA LQEPPWSLGC TGLKAAMQIQ RVVIPVPTLG HRNPWVARDS AVPARDPAWL
181QEDKVEEEAM APGLPTACSQ EPVTFADVAV VFTPEEWVFL DSTQRSLYRD VMLENYRNLA
241SVADQLCKPN ALSYLEERGE QWTTDRGVLS DTCAEPQCQP QEAIPSQDTF TEILSIDVKG
301EQPQPGEKLY KYNELEKPFN SIEPLFQYQR IHAGEASCEC QEIRNSFFQS AHLIVPEKIR
361SGDKSYACNK CEKSFRYSSD LIRHEKTHTA EKCFDCQECG QAFKYSSNLR RHMRTHTGEK
421PFECSQCGKT FTRNFNLILH QRNHTGEKPY ECKDCGKAFN QPSSLRSHVR THTGEKPFEC
481SQCGKAFREH SSLKTHLRTH TREKPYECNQ CGKPFRTSTH LNVHKRIHTG EKLYECATCG
541QVLSRLSTLK SHMRTHTGEK PYVCQECGRA FSEPSSLRKH ARTHSGKKPY ACQECGRAFG
601QSSHLIVHVR THSAGRPYQC NQCEKAFRHS SSLTVHKRTH VGRETIRNGS LPLSMSHPYC
661GPLAN
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

665 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.