Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
ZNF324 / ZF5128 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G114014-100
Catalog Number: LS-G114014
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ZNF324 (NM_014347), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Zinc finger protein 324 | Zinc finger protein 324A | ZNF324A | ZF5128 | Zinc finger protein ZF5128 | ZNF324
- Origin Species
- Human
- NCBI GenBank
- NM_014347
- SwissProt
- Q6AW86
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 60.9 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MAFEDVAVYF SQEEWGLLDT AQRALYRRVM LDNFALVASL GLSTSRPRVV IQLERGEEPW61VPSGTDTTLS RTTYRRRNPG SWSLTEDRDV SGEWPRAFPD TPPGMTTSVF PVAGACHSVK121SLQRQRGASP SRERKPTGVS VIYWERLLLG SGSGQASVSL RLTSPLRPPE GVRLREKTLT181EHALLGRQPR TPERQKPCAQ EVPGRTFGSA QDLEAAGGRG HHRMGAVWQE PHRLLGGQEP241STWDELGEAL HAGEKSFECR ACSKVFVKSS DLLKHLRTHT GERPYECAQC GKAFSQTSHL301TQHQRIHSGE TPYACPVCGK AFRHSSSLVR HQRIHTAEKS FRCSECGKAF SHGSNLSQHR361KIHAGGRPYA CAQCGRRFCR NSHLIQHERT HTGEKPFVCA LCGAAFSQGS SLFKHQRVHT421GEKPFACPQC GRAFSHSSNL TQHQLLHTGE RPFRCVDCGK AFAKGAVLLS HRRIHTGEKP481FVCTQCGRAF RERPALFHHQ RIHTGEKTVR RSRASLHPQA RSVAGASSEG APAKETEPTP541ASGPAAVSQP AEVSGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
553 aa + 34 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.