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ZNF320 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ZNF320 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G102753-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ZNF320 (NM_207333), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Zinc finger protein 320 | Zinc finger protein like | ZFPL | Zinc finger gene 320 | ZNF320
Origin Species
Human
SwissProt
A2RRD8

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
59.1 kDa
Protein Type
Lysate
Amino Acid Sequence
1MALSQGLLTF RDVAIEFSQE EWKCLDPAQR TLYRDVMLEN YRNLVSLDIS SKCMMNTLSS
61TGQGNTEVIH TGTLQRQASY HIGAFCSQEI EKDIHDFVFQ WQEDETNDHE APMTEIKKLT
121SSTDRYDQRH AGNKPIKGQL ESRFHLHLRR HRRIHTGEKP YKCEECEKVF SCKSHLEIHR
181IIHTGEKPYK CKVCDKAFKH DSHLAKHTRI HRGDKHYTCN ECGKVFDQKA TLACHHRSHT
241GEKPYKCNEC GKTFSQTSHL VYHHRLHTGE KPYKCNECGK TFARNSVLVI HKAVHTAEKP
301YKCNECGKVF KQRATLAGHR RVHTGEKPYR CEECDKVFSR KSHLERHRRI HTGEKPYKCK
361VCDKAFRSDS RLAEHQRVHT GERPYTCNEC GKVFSTKAYL ACHQKLHTGE KLYECEECDK
421VYIRKSHLER HRRIHTGEKP HKCGDCGKAF NSPSHLIRHQ RIHTGQKSYK CHQCGKVFSL
481RSLLAEHQKI PFGDNCFKCN EYSKPSSIN
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

509 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.