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ZNF319 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ZNF319 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G110963-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ZNF319 (NM_020807), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
ZFP319 | Zinc finger protein 319 | KIAA1388 | ZNF319
Origin Species
Human
SwissProt
Q9P2F9

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
65.5 kDa
Protein Type
Lysate
Amino Acid Sequence
1MSESWQQPPQ TQPQQPQPPQ PQHHAEPPPA LAEHTLPPGT AENPLGCAVY GILLQPDPGL
61QPPQHAPLQA AGEPGPKCGV CGHDLAHLSS PHEHQCLAGH DRSFQCTQCL KIFHQATDLL
121EHQCVQAEQK PFVCGVCKMG FSLLTSLAQH HSSHSGLVKC SICEKTYKPA EAAEPATTAA
181PSLPAAPAPS TVTPAEQADK PYSCPICQKP FKHLSELSRH ERIHTGEKPY KCTLCDKSFS
241QSSHLVHHKR THSSERPYKC AVCEKTFKHR SHLVRHMYAH SGEHHLFRCN VCELHFKESS
301ELLQHPCTPS GERPFRCGEC QKAFKRPSDL RQHERTHSAE RPFKCDLCPM GFKQQYALMR
361HRRTHKTEEP FKCGLCEKGF GQPSHLLYHQ HVHTLETLFK CPVCQKGFDQ SAELLRHKCL
421PGAAERPFKC PVCNKAYKRA SALQKHQLAH CAAAEKPLRC TLCERRFFSS SEFVQHRCDP
481AREKPLKCPD CEKRFKYASD LQRHRRVHTG EKPYKCPNCD KAFKQREHLN KHQGVHAREQ
541QFKCVWCGER FLDVALLQEH SAQHSAAAAA AEGAYQVAAC LP
SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV

582 aa + 34 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.