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ZNF302 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ZNF302 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G121829-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ZNF302 (NM_001012320), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
MST154 | HSD16 | MSTP154 | Zinc finger protein 302 | Zinc finger protein 327 | Zinc finger protein 135-like | Zinc finger protein 140-like | ZNF135L | ZNF140L | ZNF302 | ZNF327
Origin Species
Human
NCBI GenBank
NM_018443
SwissProt
Q9NR11

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
45.8 kda
Protein Type
Lysate
Amino Acid Sequence
1MSQVTFSDVA IDFSHEEWAC LDSAQRDLYK DVMVQNYENL VSVGLSVTKP YVIMLLEDGK
61EPWMMEKKLS KDWESRWENK ELSTKKDIYD EDSPQPVTME KVVKQSYEFS NSNKNLEYTE
121CDTFRSTFHS KSTLSEPQNN SAEGNSHKYD ILKKNLSKKS VIKSERINGG KKLLNSNKSG
181AAFNQSKSLT LPQTCNREKI YTCSECGKAF GKQSILSRHW RIHTGEKPYE CRECGKTFSH
241GSSLTRHQIS HSGEKPYKCI ECGKAFSHGS SLTNHQSTHT GEKPYECMNC GKSFSRVSLL
301IQHLRIHTQE KRYECRICGK AFIHSSSLIH HQKSHTGEKP YECRECGKAF CCSSHLTQHQ
361RIHSMKKKYE CNKCLKVFSS FSFLVQHQSI HTEEKPFEV
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

399 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.