LSBio
ZNF295 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ZNF295 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all ZBTB21 antibodies →
$725.00each
Catalog Number: LS-G119198-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ZNF295(ZBTB21) (NM_001098403), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
ZBTB21 | ZNF295
Origin Species
Human
NCBI GenBank
NM_020727
SwissProt
Q9ULJ3

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
95.6 kda
Protein Type
Lysate
Amino Acid Sequence
1MEGLLHYINP AHAISLLSAL NEERLKGQLC DVLLIVGDQK FRAHKNVLAA SSEYFQSLFT
61NKENESQTVF QLDFCEPDAF DNVLNYIYSS SLFVEKSSLA AVQELGYSLG ISFLTNIVSK
121TPQAPFPTCP NRKKVFVEDD ENSSQKRSVI VCQSRNEAQG KTVSQNQPDV SHTSRPSPSI
181AVKANTNKPH VPKPIEPLHN LSLTEKSWPK DSSVVYAKSL EHSGSLDDPN RISLVKRNAV
241LPSKPLQDRE AMDDKPGVSG QLPKGKALEL ALKRPRPPVL SVCSSSETPY LLKETNKGNG
301QGEDRNLLYY SKLGLVIPSS GSGSGNQSID RSGPLVKSLL RRSLSMDSQV PVYSPSIDLK
361SSQGSSSVSS DAPGNVLCAL SQKSSLKDCS EKTALDDRPQ VLQPHRLRSF SASQSTDREG
421ASPVTEVRIK TEPSSPLSDP SDIIRVTVGD AATTAAASSS SVTRDLSLKT EDDQKDMSRL
481PAKRRFQADR RLPFKKLKVN EHGSPVSEDN FEEGSSPTLL DADFPDSDLN KDEFEQGSHE
541RLCRNAAVCP YCSLRFFSPE LKQEHESKCE YKKLTCLECM RTFKSSFSIW RHQVEVHNQN
601NMAPTENFSL PVLDHNGDVT GSSRPQSQPE PNKVNHIVTT KDDNVFSDSS EQVNFDSEDS
661SCLPEDLSLS KQLKIQVKEE PVEEAEEEAP EASTAPKEAG PSKEASLWPC EKCGKMFTVH
721KQLERHQELL CSVKPFICHV CNKAFRTNFR LWSHFQSHMS QASEESAHKE SEVCPVPTNS
781PSPPPLPPPP PLPKIQPLEP DSPTGLSENP TPATEKLFVP QESDTLFYHA PPLSAITFKR
841QFMCKLCHRT FKTAFSLWSH EQTHN
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

865 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.