LSBio
ZNF287 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ZNF287 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G110999-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Zinc finger protein 287(ZNF287) (NM_020653), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Zinc finger protein 287 | ZKSCAN13 | ZNF287 | ZSCAN45
Origin Species
Human
NCBI GenBank
NM_020653
SwissProt
Q9HBT7

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
87.4 kDa
Protein Type
Lysate
Amino Acid Sequence
1MNSSSRSQIL LRWKSDKAQS GPYNVEKEIL TSRFLRDTET CRQNFRNFPY PDLAGPRKAL
61SQLRELCLKW LRPEIHSKEQ ILELLVLEQF LTILPGEVRT WVKSQYPESS EEAVTLVEDL
121TQILEEEAPQ NSTLSQDTPE EDPRGKHAFQ TGWLNDLVTK ESMTFKDVAV DITQEDWELM
181RPVQKELYKT VTLQNYWNMV SLGLTVYRPT VIPILEEPWM VIKEILEGPS PEWETKAQAC
241TPVEDMSKLT KEETHTIKLE DSYDYDDRLE RRGKGGFWKI HTDERGFSLK SVLSQEYDPT
301EECLSKYDIY RNNFEKHSNL IVQFDTQLDN KTSVYNEGRA TFNHVSYGIV HRKILPGEKP
361YKCNVCGKKF RKYPSLLKHQ STHAKEKSYE CEECGKEFRH ISSLIAHQRM HTGEKPYECH
421QCGKAFSQRA HLTIHQRIHT GEKPYKCDDC GKDFSQRAHL TIHQRTHTGE KPYKCLECGK
481TFSHSSSLIN HQRVHTGEKP YICNECGKTF SQSTHLLQHQ KIHTGKKPYK CNECWKVFSQ
541STYLIRHQRI HSGEKCYKCN ECGKAFAHSS TLIQHQTTHT GEKSYICNIC GKAFSQSANL
601TQHHRTHTGE KPYKCSVCGK AFSQSVHLTQ HQRIHNGEKP FKCNICGKAY RQGANLTQHQ
661RIHTGEKPYK CNECGKAFIY SSSLNQHQRT HTGERPYKCN ECDKDFSQRT CLIQHQRIHT
721GEKPYACRIC GKTFTQSTNL IQHQRVHTGA KHRN
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

754 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.