LSBio
ZNF286B Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ZNF286B Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all ZNF286B antibodies →
$504.00each
Catalog Number: LS-G126493-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ZNF286B (NM_001145045), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
ZNF286C | Zinc finger protein 286-like | Zinc finger protein 286B | ZNF590 | Zinc finger protein 590 | ZNF286B | ZNF286L | Zinc finger 286C pseudogene
Origin Species
Human
NCBI GenBank
NM_001145045
SwissProt
P0CG31

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
59.4 kda
Protein Type
Lysate
Amino Acid Sequence
1METDLAEMPE KGVLSSQDSP HFQEKSTEEG EVAALRLTAR SQAAAAAAAP GSRSLRGVHV
61PPPLHPAPAR EEIKSTCSLK ACFSLSLTLT YYRTAFLLST ENEGNLHFQC PSDVETRPQS
121KDSTSVQDFS KAESCKVAII DRLTRNSVYD SNLEAALECE NWLEKQQGNQ ERHLREMFTH
181MNSLSEETDH EHDVYWKSFN QKSVLITEDR VPKGSYAFHT LEKSLKQKSN LMKKQRTYKE
241KKPHKCNDCG ELFTCHSVHI QHQRVHTGEK PYTCNECGKS FSHRANLTKH QRTHTRILFE
301CRECKKTFTE SSSLATHQRI HVGERPYECN ECGKGFNRST HLVQHQLIHT GVRPYECNEC
361DKAFIHSSAL IKHQRTHTGE KPYKCQECGK AFSHCSSLTK HQRVHTGEKP YECSECGKTF
421SQSTHLVQHQ RIHTGEKPYE CSECGKTFSQ SSNFAKHQRI HIGKKPYKCS ECGKAFIHSS
481ALIQHQRTHT GEKPFRCNEC GKSFKCSSSL IRHQRVHTEE QP
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

522 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.