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ZNF286A Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ZNF286A Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G110998-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ZNF286(ZNF286A) (NM_020652), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
KIAA1874 | Zinc finger protein 286A | Zinc finger protein 286 | ZNF286A | Zinc finger protein ZNF286 | ZNF286
Origin Species
Human
NCBI GenBank
NM_001130842

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
60 kda
Protein Type
Lysate
Amino Acid Sequence
1METDLAEMPE KGALSSQDSP HFQEKSTEEG EVAALRLTAR SQETVTFKDV AMDFTPEEWG
61KLDPAQRDVM LENYRNLVSL WLPVSKPESY NLENGKEPLK LERKAPKSSY SDMETRPQSK
121DSTSVQDFSK AESCKVAIID RLTRNSVYDS NLEAALECEN WLENQQGNQE RHLREMFTHM
181NSLSEETDHK HDVYWKSFNQ KSVLITEDRV PKGSYAFHTL EKSLKQKSNL MKKQRTYKEK
241KPHKCNDCGE LFTYHSVLIR HQRVHTGEKP YTCNECGKSF SHRANLTKHQ RTHTRILFEC
301SECKKTFTES SSLATHQRIH VGERPYECNE CGKGFNRSTH LVQHQLIHTG VKPYECNECD
361KAFIHSSALI KHQRTHTGEK PYKCQECGKA FSHCSSLTKH QRVHTGEKPY ECSECGKTFS
421QSTHLVQHQR IHTGEKPYEC NECGKTFSRS SNFAKHQRIH IGKKPYKCSE CGKAFIHSSA
481LIQHQRTHTG EKPFRCNECG KSFKCSSSLI RHQRVHTEEQ P
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

521 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.