Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
ZNF285 / ZNF285A Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G106327-100
Catalog Number: LS-G106327
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ZNF285 (NM_152354), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Zinc finger protein 285A | ZNF285A | Zinc finger protein 285 | ZNF285
- Origin Species
- Human
- SwissProt
- Q96NJ3
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 68.1 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MIKFQERVTF KDVAVVFTKE ELALLDKAQI NLYQDVMLEN FRNLMLVRDG IKNNILNLQA61KGLSYLSQEV LHCWQIWKQR IRDLTVSQDY IVNLQEECSP HLEDVSLSEE WAGISLQISE121NENYVVNAII KNQDITAWQS LTQVLTPESW RKANIMTEPQ NSQGRYKGIY MEEKLYRRAQ181HDDSLSWTSC DHHESQECKG EDPGRHPSCG KNLGMKSTVE KRNAAHVLPQ PFPCNNCGVA241FADDTDPHVH HSTHLGEKSY KCDQYGKNFS QSQDLIVHCK THSGKTPYEF HEWPMGCKQS301SDLPRYQKVS SGDKPYKCKE CGKGFRRSSS LHNHHRVHTG EMPYKCDECG KGFGFRSLLC361IHQGVHTGKK PYKCEECGKG FDQSSNLLVH QRVHTGEKPY KCSECGKCFS SSSVLQVHWR421FHTGEKPYRC GECGKGFSQC THLHIHQRVH TGEKPYKCNV CGKDFAYSSV LHTHQRVHTG481EKPYKCEVCG KCFSYSSYFH LHQRDHIREK PYKCDECGKG FSRNSDLNVH LRVHTGERPY541KCKACGKGFS RNSYLLAHQR VHIDETQYTH CERGKDLLTH QRLHEQRETLTRTRPLEQKLISEEDLAANDILDYKDDDDKV
590 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.