LSBio
ZNF283 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ZNF283 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G104310-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ZNF283 (NM_181845), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
HZF19 | HZF41 | Zinc finger protein HZF19 | Zinc finger protein 41 | Zinc finger protein 283 | ZNF283
Origin Species
Human
NCBI GenBank
NM_181845
SwissProt
Q8N7M2

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
77.8 kDa
Protein Type
Lysate
Amino Acid Sequence
1MESRSVAQAG VQWCDLGSLQ APPPGFTLFS CLSLLSSWDY SSGFSGFCAS PIEESHGALI
61SSCNSRTMTD GLVTFRDVAI DFSQEEWECL DPAQRDLYVD VMLENYSNLV SLDLESKTYE
121TKKIFSENDI FEINFSQWEM KDKSKTLGLE ASIFRNNWKC KSIFEGLKGH QEGYFSQMII
181SYEKIPSYRK SKSLTPHQRI HNTEKSYVCK ECGKACSHGS KLVQHERTHT AEKHFECKEC
241GKNYLSAYQL NVHQRFHTGE KPYECKECGK TFSWGSSLVK HERIHTGEKP YECKECGKAF
301SRGYHLTQHQ KIHTGVKSYK CKECGKAFFW GSSLAKHEII HTGEKPYKCK ECGKAFSRGY
361QLTQHQKIHT GKKPYECKIC GKAFCWGYQL TRHQIFHTGE KPYECKECGK AFNCGSSLIQ
421HERIHTGEKP YECKECGKAF SRGYHLSQHQ KIHTGEKPFE CKECGKAFSW GSSLVKHERV
481HTGEKSHECK ECGKTFCSGY QLTRHQVFHT GEKPYECKEC GKAFNCGSSL VQHERIHTGE
541KPYECKECGK AFSRGYHLTQ HQKIHTGEKP FKCKECGKAF SWGSSLVKHE RVHTNEKSYE
601CKDCGKAFGS GYQLSVHQRF HTGEKLYQRK EFGKTFTCGS KLVHERTHSN DKPYKYNECG
661EAFLWTTYSN EKIDTDETL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

679 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.