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ZNF282 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ZNF282 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G117238-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ZNF282 (NM_003575), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
HUB1 | Zinc finger protein 282 | ZNF282 | HTLV-I U5RE-binding protein 1 | HUB-1
Origin Species
Human
NCBI GenBank
D30612
SwissProt
Q9UDV7

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
74.1 kDa
Protein Type
Lysate
Amino Acid Sequence
1MQFVSTRPQP QQLGIQGLGL DSGSWSWAQA LPPEEVCHQE PALRGEMAEG MPPMQAQEWD
61MDARRPMPFQ FPPFPDRAPV FPDRMMREPQ LPTAEISLWT VVAAIQAVER KVDAQASQLL
121NLEGRTGTAE KKLADCEKTA VEFGNHMESK WAVLGTLLQE YGLLQRRLEN LENLLRNRNF
181WVLRLPPGSK GEAPKVPVTF VDIAVYFSED EWKNLDEWQK ELYNNLVKEN YKTLMSLDAE
241GSVPKPDAPV QAEPREEPCV WEQRHPEERE IPMDPEAGAE PLVPAQDASS QVKREDTLCV
301RGQRGLEERA IPTESITDSP ISAQDLLSRI KQEEHQCVWD QQDLADRDIP TDPNSESLIS
361AHDILSWIKQ EEQPYPWGPR DSMDGELGLD SGPSDSLLMV KNPPPAPPQP QPQPQPPQPQ
421LQSQPQPQSL PPIAVAENPG GPPSRGLLDD GFQVLPGERG SGEAPPGGDR STGGGGGDGG
481GGGGGAEAGT GAGGGCGSCC PGGLRRSLLL HGARSKPYSC PECGKSFGVR KSLIIHHRSH
541TKERPYECAE CEKSFNCHSG LIRHQMTHRG ERPYKCSECE KTYSRKEHLQ NHQRLHTGER
601PFQCALCGKS FIRKQNLLKH QRIHTGERPY TCGECGKSFR YKESLKDHLR VHSGGPGPGA
661PRQLPPPPER D
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

671 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.