Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
ZNF281 / Zfp281 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G114392-100
Catalog Number: LS-G114392
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ZNF281 (NM_012482), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- GZP1 | ZBP99 | Zfp281 | ZNP-99 transcription factor | Zinc finger protein 281 | ZNF281 | ZNP-99 | Transcription factor ZBP-99 | ZBP-99
- Origin Species
- Human
- NCBI GenBank
- NM_012482
- SwissProt
- Q9Y2X9
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 96.7 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MKIGSGFLSG GGGTGSSGGS GSGGGGSGGG GGGGSSGRRA EMEPTFPQGM VMFNHRLPPV61TSFTRPAGSA APPPQCVLSS STSAAPAAEP PPPPAPDMTF KKEPAASAAA FPSQRTSWGF121LQSLVSIKQE KPADPEEQQS HHHHHHHHYG GLFAGAEERS PGLGGGEGGS HGVIQDLSIL181HQHVQQQPAQ HHRDVLLSSS SRTDDHHGTE EPKQDTNVKK AKRPKPESQG IKAKRKPSAS241SKPSLVGDGE GAILSPSQKP HICDHCSAAF RSSYHLRRHV LIHTGERPFQ CSQCSMGFIQ301KYLLQRHEKI HSREKPFGCD QCSMKFIQKY HMERHKRTHS GEKPYKCDTC QQYFSRTDRL361LKHRRTCGEV IVKGATSAEP GSSNHTNMGN LAVLSQGNTS SSRRKTKSKS IAIENKEQKT421GKTNESQISN NINMQSYSVE MPTVSSSGGI IGTGIDELQK RVPKLIFKKG SRKNTDKNYL481NFVSPLPDIV GQKSLSGKPS GSLGIVSNNS VETIGLLQST SGKQGQISSN YDDAMQFSKK541RRYLPTASSN SAFSINVGHM VSQQSVIQSA GVSVLDNEAP LSLIDSSALN AEIKSCHDKS601GIPDEVLQSI LDQYSNKSES QKEDPFNIAE PRVDLHTSGE HSELVQEENL SPGTQTPSND661KASMLQEYSK YLQQAFEKST NASFTLGHGF QFVSLSSPLH NHTLFPEKQI YTTSPLECGF721GQSVTSVLPS SLPKPPFGML FGSQPGLYLS ALDATHQQLT PSQELDDLID SQKNLETSSA781FQSSSQKLTS QKEQKNLESS TGFQIPSQEL ASQIDPQKDI EPRTTYQIEN FAQAFGSQFK841SGSRVPMTFI TNSNGEVDHR VRTSVSDFSG YTNMMSDVSE PCSTRVKTPT SQSYRTRTRPLEQKLISEEDLAANDILDYKDDDDKV
895 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.