LSBio
ZNF280D Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ZNF280D Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all ZNF280D antibodies →
$725.00each
Catalog Number: LS-G112333-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ZNF280D (NM_017661), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
KIAA1584 | SUHW4 | ZNF634 | Zinc finger protein 280D | ZNF280D | Zinc finger protein 634
Origin Species
Human
NCBI GenBank
AK000093
SwissProt
Q6N043

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
109.1 kDa
Protein Type
Lysate
Amino Acid Sequence
1MGDNPFQPKS NSKMAELFME CEEEELEPWQ KKVKEVEDDD DDEPIFVGEI SSSKPAISNI
61LNRVNPSSYS RGLKNGALSR GITAAFKPTS QHYTNPTSNP VPASPINFHP ESRSSDSSVI
121VQPFSKPGYI TNSSRVVSNK SSELLFDLTQ DTGLSHYQGG PTLSMAGMSE SSFLSKRPST
181SEVNNVNPKK PKPSESVSGA NSSAVLPSVK SPSVTSSQAM LAKGTNTSSN QSKNGTPFPR
241ACPKCNIHFN LLDPLKNHMK YCCPDMINNF LGLAKTEFSS TVNKNTTIDS EKGKLIMLVN
301DFYYGKHEGD VQEEQKTHTT FKCFSCLKIL KNNIRFMNHM KHHLELEKQS SESWENHTTC
361QHCYRQFPTP FQLQCHIEST HTPHEFSTIC KICELSFETE HVLLQHMKDN HKPGEMPYVC
421QVCNYRSSSF SDVETHFRTS HENTKNLLCP FCLKVIKIAT PYMHHYMKHQ KKGIHRCTKC
481RLQFLTCKEK MDHKTQHHRT FIKPKQLEGL PPGTKVTIRA SVGPLQSGAS PTPSISASAS
541TLQLSPPRTK NITAKNPAKS NTSKPNTVKS NASKPNTSKP NGSKSKYKPK ISNMQKKQST
601LASSNKKSKV NTALRNLRYR RGIHKCIECC SEIKDFANHF PTYVHCSFCR YNTSCSKAYV
661NHMMSFHSNR PSKRFCIFKK HSENLRGITL VCLNCDFLSD VSGLDNMATH LSQHKTHTCQ
721VVMQKVSVCI PTSEHLSELK KEAPAKEQEP VSKEIARPNM AERETETSNS ESKQDKAASS
781KEKNGCNANS FEGSSTTKSE ESITVSDKEN ETCLADQETG SKNIVSCDSN IGADKVEKKK
841QIQHVCQEME LKMCQSSENI ILSDQIKDHN SSEARFSSKN IKDLRLASDN VSIDQFLRKR
901HEPESVSSDV SEQGSIHLEP LTPSEVLEYE ATEILQKGSG DPSAKTDEVV SDQTDDIPGG
961NNPSTTEATV DLEDEKERS
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

979 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.