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ZNF280B Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ZNF280B Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G107779-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SUHW2(ZNF280B) (NM_080764), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
5OY11.1 | 5'OY11.1 | SUHW2 | Zinc finger protein 279 | ZNF280B | ZNF632 | Zinc finger protein 632 | D87009.C22.3 | Zinc finger protein 280B | ZNF279
Origin Species
Human
SwissProt
Q86YH2

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
61.4 kDa
Protein Type
Lysate
Amino Acid Sequence
1MEQSCEEEKE PEPQKNIQET KQVDDEDAEL IFVGVEHVNE DAELIFVGVT SNSKPVVSNI
61LNRVTPGSWS RRKKYDHLRK DTARKLQPKS HETVTSEAVT VLPASQLESR STDSPIIIEP
121LSKPDYRNSS PQVVPNNSSE LPSPLITFTD SLHHPVSTAL SVGGINESPR VSKQLSTFEV
181NSINPKRAKL RDGIIEGNSS ASFPSDTFHT MNTQQSTPSN NVHTSLSHVQ NGAPFPAAFP
241KDNIHFKPIN TNLDRENELA KTDILSLTSQ NKTFDPKKEN PIVLLSDFYY GQHKGEGQPE
301QKTHTTFKCL SCVKVLKNVK FMNHVKHHLE FEKQRNDSWE NHTTCQHCHR QFPTPFQLQC
361HIENVHTAQE PSTVCKICEL SFETDQVLLQ HMKDHHKPGE MPYVCQVCHY RSSVFADVET
421HFRTCHENTK NLLCPFCLKI FKTATPYMCH YRGHWGKSAH QCSKCRLQFL TFKEKMEHKT
481QCHQMFKKPK QLEGLPPETK VTIQVSLEPL QPGSVDVASI TVSTSDSEPS LPRSKSKISK
541KSH
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

543 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.