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ZNF280A Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ZNF280A Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G107763-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SUHW1(ZNF280A) (NM_080740), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
3OY11.1 | 3'OY11.1 | Zinc finger protein 280 | Zinc finger protein 280A | ZNF280 | ZNF636 | ZNF280A | SUHW1 | Zinc finger protein 636
Origin Species
Human
SwissProt
P59817

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
60.6 kDa
Protein Type
Lysate
Amino Acid Sequence
1MGDIFLCKKV ESPKKNLRES KQREEDDEDP DLIYVGVEHV HRDAEVLFVG MISNSKPVVS
61NILNRVTPGS NSRRKKGHFR QYPAHVSQPA NHVTSMAKAI MPVSLSEGRS TDSPVTMKSS
121SEPGYKMSSP QVVSPSSSDS LPPGTQCLVG AMVSGGGRNE SSPDSKRLST SDINSRDSKR
181VKLRDGIPGV PSLAVVPSDM SSTISTNTPS QGICNSSNHV QNGVTFPWPD ANGKAHFNLT
241DPERASESAL AMTDISSLAS QNKTFDPKKE NPIVLLSDFY YGQHKGDGQP EQKTHTTFKC
301LSCVKVLKNI KFMNHMKHHL EFEKQRNDSW EDHTTCQHCH RQFPTPFQLQ CHIDSVHIAM
361GPSAVCKICE LSFETDQVLL QHMKDHHKPG EMPYVCQVCH YRSSVFADVE THFRTCHENT
421KNLLCLFCLK LFKTAIPYMN HCWRHSRRRV LQCSKCRLQF LTLKEEIEHK TKDHQTFKKP
481EQLQGLPSET KVIIQTSVQP GSSGMASVIV SNTDPQSSPV KTKKKTAMNT RDSRLPCSKD
541SS
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

542 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.