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ZNF276 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ZNF276 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all ZNF276 antibodies →
$725.00each
Catalog Number: LS-G106392-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ZNF276 (NM_152287), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
CENP-Z | Centromere protein Z | Zinc finger protein 276 | ZNF477 | ZFP276 | Zfp-276 | ZNF276 | CENPZ | Zinc finger protein 477
Origin Species
Human
SwissProt
Q8N554

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
59.7 kDa
Protein Type
Lysate
Amino Acid Sequence
1MGHCRLCHGK FSSRSLRSIS ERAPGASMER PSAEERVLVR DFQRLLGVAV RQDPTLSPFV
61CKSCHAQFYQ CHSLLKSFLQ RVNASPAGRR KPCAKVGAQP PTGAEEGACL VDLITSSPQC
121LHGLVGWVHG HAASCGALPH LQRTLSSEYC GVIQVVWGCD QGHDYTMDTS SSCKAFLLDS
181ALAVKWPRDK ETAPRLPQHR GWNPGDAPQT SQGRGTGTPV GAETKTLPST DVAQPPSDSD
241AVGPRSGFPP QPSLPLCRAP GQLGEKQLPS STSDDRVKDE FSDLSEGDVL SEDENDKKQN
301AQSSDESFEP YPERKVSGKK SESKEAKKSE EPRIRKKPGP KPGWKKKLRC EREELPTIHK
361CPYQGCTAVY RGADGMKKHI KEHHEEVRER PCPHPGCNKV FMIDRYLQRH VKLIHTEVRN
421YICDECGQTF KQRKHLLVHQ MRHSGAKPLQ CEVCGFQCRQ RASLKYHMTK HKAETELDFA
481CDQCGRRFEK AHNLNVHMSM VHPLTQTQDK ALPLEAEPPP GPPSPSVTTE GQAVKPEPT
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

539 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.