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ZNF275 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ZNF275 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G119643-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ZNF275 (NM_001080485), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
ZNF275 | Zinc finger protein 275
Origin Species
Human
NCBI GenBank
NM_001080485
SwissProt
Q9NSD4

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
55.38 kDa
Protein Type
Lysate
Amino Acid Sequence
1MPVVESFTYF AIWKKKIIYG KTDEPWEFFQ DLVTFDDVAW YLTTREWFKL DPEQRALEYY
61GNVTSVGVPV LNPALVPHLA QGQVLLVSDP SPNTDPAKYS ESTSATRHQM KGEDAQPQEM
121ASTSFPRASG PSPEFRQHGD SDGKRGSPQN LPIEHHFACK ECGDTFRLKV LLVQHQRVHS
181EEKGWECGDC GKVFRGVAEF NEHRKSHVAA EPQPGPSRAL ENAAEKREQM EREAKPFECE
241ECGKRFKKNA GLSQHLRVHS REKPFDCEEC GRSFKVNTHL FRHQKLHTSE KPFACKACSR
301DFLDRQELLK HQRMHTGHLP FDCDDCGKSF RGVNGLAEHQ RIHSGAKPYG CPHCGKLFRR
361SSELTKHRRI HTGEKPYACG QCGKAFRQSS SLLEHARIHS GERPYACGEC GKAFRGPSDL
421IKHRRIHSGL KPYECDKCGK AFRRSSGLSR HRRIHSGARR CECSQCGRVF KRRSALQKHQ
481PTHHE
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

485 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.