LSBio
ZNF274 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ZNF274 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all ZNF274 antibodies →
$504.00each
Catalog Number: LS-G107536-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ZNF274 (NM_133502), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
HFB101 | ZF2 | Zinc finger protein 274 | Zinc finger protein zfp2 | ZNF274 | ZSCAN51 | Zinc finger protein HFB101 | ZKSCAN19
Origin Species
Human
NCBI GenBank
NM_016325
SwissProt
Q96GC6

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
74 kDa
Protein Type
Lysate
Amino Acid Sequence
1MASRLPTAWS CEPVTFEDVT LGFTPEEWGL LDLKQKSLYR EVMLENYRNL VSVEHQLSKP
61DVVSQLEEAE DFWPVERGIP QDTIPEYPEL QLDPKLDPLP AESPLMNIEV VEVLTLNQEV
121AGPRNAQIQA LYAEDGSLSA DAPSEQIQQQ GKHPGDPEAA RQRFRQFRYK DMTGPREALD
181QLRELCHQWL QPKARSKEQI LELLVLEQFL GALPVKLRTW VESQHPENCQ EVVALVEGVT
241WMSEEEVLPA GQPAEGTTCC LEVTAQQEEK QEDAAICPVT VLPEEPVTFQ DVAVDFSREE
301WGLLGPTQRT EYRDVMLETF GHLVSVGWET TLENKELAPN SDIPEEEPAP SLKVQESSRD
361CALSSTLEDT LQGGVQEVQD TVLKQMESAQ EKDLPQKKHF DNRESQANSG ALDTNQVSLQ
421KIDNPESQAN SGALDTNQVL LHKIPPRKRL RKRDSQVKSM KHNSRVKIHQ KSCERQKAKE
481GNGCRKTFSR STKQITFIRI HKGSQVCRCS ECGKIFRNPR YFSVHKKIHT GERPYVCQDC
541GKGFVQSSSL TQHQRVHSGE RPFECQECGR TFNDRSAISQ HLRTHTGAKP YKCQDCGKAF
601RQSSHLIRHQ RTHTGERPYA CNKCGKAFTQ SSHLIGHQRT HNRTKRKKKQ PTS
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

653 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.