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ZNF264 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ZNF264 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G117365-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ZNF264 (NM_003417), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
KIAA0412 | Zinc finger protein 264 | ZNF264
Origin Species
Human
NCBI GenBank
AB007872
SwissProt
O43296

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
70.4 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAAAVLTDRA QVSVTFDDVA VTFTKEEWGQ LDLAQRTLYQ EVMLENCGLL VSLGCPVPKA
61ELICHLEHGQ EPWTRKEDLS QDTCPGDKGK PKTTEPTTCE PALSEGISLQ GQVTQGNSVD
121SQLGQAEDQD GLSEMQEGHF RPGIDPQEKS PGKMSPECDG LGTADGVCSR IGQEQVSPGD
181TVHSHNSCES GKDPMIQEEE NNFKCSECGK VFNKKHLLAG HEKIHSGVKP YECTECGKTF
241IKSTHLLQHH MIHTGERPYE CMECGKAFNR KSYLTQHQRI HSGEKPYKCN ECGKAFTHRS
301NFVLHNRRHT GEKSFVCTEC GQVFRHRPGF LRHYVVHSGE NPYECLECGK VFKHRSYLMW
361HQQTHTGEKP YECSECGKVF LESAALIHHY VIHTGEKPFE CLECGKAFNH RSYLKRHQRI
421HTGEKPFVCS ECGKAFTHCS TFILHKRAHT GEKPFECKEC GKAFSNRKDL IRHFSIHTGE
481KPYECVECGK AFTRMSGLTR HKRIHSGEKP YECVECGKSF CWSTNLIRHA IIHTGEKPYK
541CSECGKAFSR SSSLTQHQRM HTGKNPISVT DVGRPFTSGQ TSVTLRELLL GKDFLNVTTE
601ANILPEETSS SASDQPYQRE TPQVSSL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

627 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.