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ZNF238 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ZNF238 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all ZBTB18 antibodies →
$725.00each
Catalog Number: LS-G103038-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ZNF238(ZBTB18) (NM_205768), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
58 kDa repressor protein | C2H2-171 | RP58 | Transcriptional repressor RP58 | Zinc finger protein 238 | Zinc finger protein C2H2-171 | ZBTB18 | TAZ-1 | TAZ1 | ZNF238
Origin Species
Human
NCBI GenBank
NM_006352
SwissProt
Q99592

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
59.2 kDa
Protein Type
Lysate
Amino Acid Sequence
1MCPKGYEDSM EFPDHSRHLL QCLSEQRHQG FLCDCTVLVG DAQFRAHRAV LASCSMYFHI
61FYKDQLDKRD IVHLNSDIVT APAFALLLEF MYEGKLQFKD LPIEDVLAAA SYLHMYDIVK
121VCKKKLKEKA TTEADSTKKE EDASSCSDKV ESLSDGSSHI AGDLPSDEDE GEDEKLNILP
181SKRDLAAEPG NMWMRLPSDS AGIPQAGGEA EPHATAAGKT VASPCSSTES LSQRSVTSVR
241DSADVDCVLD LSVKSSLSGV ENLNSSYFSS QDVLRSNLVQ VKVEKEASCD ESDVGTNDYD
301MEHSTVKESV STNNRVQYEP AHLAPLREDS VLRELDREDK ASDDEMMTPE SERVQVEGGM
361ESSLLPYVSN ILSPAGQIFM CPLCNKVFPS PHILQIHLST HFREQDGIRS KPAADVNVPT
421CSLCGKTFSC MYTLKRHERT HSGEKPYTCT QCGKSFQYSH NLSRHAVVHT REKPHACKWC
481ERRFTQSGDL YRHIRKFHCE LVNSLSVKSE ALSLPTVRDW TLEDSSQELW K
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

531 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.