LSBio
ZNF234 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ZNF234 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all ZNF234 antibodies →
$504.00each
Catalog Number: LS-G115181-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ZNF234 (NM_006630), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Origin Species
Human
NCBI GenBank
NM_006630

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
80.4 kDa
Protein Type
Lysate
Amino Acid Sequence
1MTTFKEGLTF KDVAVVFTEE ELGLLDPVQR NLYQDVMLEN FRNLLSVGHH PFKHDVFLLE
61KEKKLDIMKT ATQRKGKSAD KIQSEVETVP EAGRHEELYW GQIWKQIASD LIKYEDSMIS
121ISRFPRQGDL SCQVRAGLYT THTGQKFYQC DEYKKSFTDV FNFDLHQQLH SGEKSHTCDE
181CGKSFCYISA LHIHQRVHMG EKCYKCDVCG KEFSQSSHLQ THQRVHTVEK PFKCVECGKG
241FSRRSTLTVH CKLHSGEKPY NCEECGRAFI HASHLQEHQR IHTGEKPFKC DTCGKNFRRR
301SALNNHCMVH TGEKPYKCED CGKCFTCSSN LRIHQRVHTG EKPYKCEECG KCFIQPSQFQ
361AHRRIHTGEK PYVCKVCGKG FIYSSSFQAH QGVHTGEKPY KCNECGKSFR MKIHYQVHLV
421VHTGEKPYKC EVCGKAFRQS SYLKIHLKAH SVQKPFKCEE CGQGFNQSSR LQIHQLIHTG
481EKPYKCEECG KGFSRRADLK IHCRIHTGEK PYNCEECGKV FSQASHLLTH QRVHSGEKPF
541KCEECGKSFS RSAHLQAHQK VHTGEKPYKC GECGKGFKWS LNLDMHQRVH TGEKPYTCGE
601CGKHFSQASS LQLHQSVHTG EKPYKCDVCG KVFSRSSQLQ YHRRVHTGEK PYKCEICGKR
661FSWRSNLVSH HKIHAAGTFY ENDENSKNIR ELSEGGSSTR
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

700 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.