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ZNF230 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ZNF230 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G115405-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ZNF230 (NM_006300), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
FDZF2 | Zinc finger protein FDZF2 | ZNF230 | Zinc finger protein 230
Origin Species
Human
NCBI GenBank
NM_006300
SwissProt
Q9UIE0

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
54.4 kDa
Protein Type
Lysate
Amino Acid Sequence
1MTTFKEAVTF KDVAVFFTEE ELGLLDPAQR KLYQDVMLEN FTNLLSVGHQ PFHPFHFLRE
61EKFWMMETAT QREGNSGGKT IAEAGPHEDC PCQQIWEQTA SDLTQSQDSI INNSHFFEQG
121DVPSQVEAGL SIIHTGQKPS QNGKCKQSFS DVAIFDPPQQ FHSGEKSHTC NECGKSFCYI
181SALRIHQRVH LREKLSKCDM RGKEFSQSSC LQTRERVHTG EKPFKCEQCG KGFRCRAILQ
241VHCKLHTGEK PYICEKCGRA FIHDFQLQKH QIIHTGEKPF KCEICGKSFC LRSSLNRHCM
301VHTAEKLYKS EECGKGFTDS LDLHKHQIIH TGQKPYNCKE CGKSFRWSSY LLIHQRIHSG
361EKPYRCEECG KGYISKSGLN LHQRVHTGER PYNCKECGKS FSRASSILNH KKLHCRKKPF
421KCEDCGKRLV HRSFCKDQQG DHNGENSSKC EDCGKRYKRR LNLDIILSLF LNDM
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

474 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.