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ZNF226 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ZNF226 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all ZNF226 antibodies →
$504.00each
Catalog Number: LS-G120857-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ZNF226 (NM_001032372), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
HZF4 | Zinc finger protein 226 | ZNF226 | Kruppel-associated box protein | ZNF269
Origin Species
Human
NCBI GenBank
NM_001032372
SwissProt
Q14588

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
91.7 kDa
Protein Type
Lysate
Amino Acid Sequence
1MNMFKEAVTF KDVAVAFTEE ELGLLGPAQR KLYRDVMVEN FRNLLSVGHP PFKQDVSPIE
61RNEQLWIMTT ATRRQGNLGE KNQSKLITVQ DRESEEELSC WQIWQQIAND LTRCQDSMIN
121NSQCHKQGDF PYQVGTELSI QISEDENYIV NKADGPNNTG NPEFPILRTQ DSWRKTFLTE
181SQRLNRDQQI SIKNKLCQCK KGVDPIGWIS HHDGHRVHKS EKSYRPNDYE KDNMKILTFD
241HNSMIHTGQK SYQCNECKKP FSDLSSFDLH QQLQSGEKSL TCVERGKGFC YSPVLPVHQK
301VHVGEKLKCD ECGKEFSQGA HLQTHQKVHV IEKPYKCKQC GKGFSRRSAL NVHCKVHTAE
361KPYNCEECGR AFSQASHLQD HQRLHTGEKP FKCDACGKSF SRNSHLQSHQ RVHTGEKPYK
421CEECGKGFIC SSNLYIHQRV HTGEKPYKCE ECGKGFSRPS SLQAHQGVHT GEKSYICTVC
481GKGFTLSSNL QAHQRVHTGE KPYKCNECGK SFRRNSHYQV HLVVHTGEKP YKCEICGKGF
541SQSSYLQIHQ KAHSIEKPFK CEECGQGFNQ SSRLQIHQLI HTGEKPYKCE ECGKGFSRRA
601DLKIHCRIHT GEKPYNCEEC GKVFRQASNL LAHQRVHSGE KPFKCEECGK SFGRSAHLQA
661HQKVHTGDKP YKCDECGKGF KWSLNLDMHQ RVHTGEKPYK CGECGKYFSQ ASSLQLHQSV
721HTGEKPYKCD VCGKVFSRSS QLQSHQRVHT GEKPYKCEIC GKSFSWRSNL TVHHRIHVGD
781KSYKSNRGGK NIRESTQEKK SIK
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

803 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.