LSBio
ZNF225 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ZNF225 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all ZNF225 antibodies →
$725.00each
Catalog Number: LS-G114330-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ZNF225 (NM_013362), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
C2-H2 type zinc finger protein | ZNF225 | Zinc finger protein 225
Origin Species
Human
NCBI GenBank
NM_013362
SwissProt
Q9UK10

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
82.3 kDa
Protein Type
Lysate
Amino Acid Sequence
1MTTLKEAVTF KDVAVVFTEE ELRLLDLAQR KLYREVMLEN FRNLLSVGHQ SLHRDTFHFL
61KEEKFWMMET ATQREGNLGG KIQMEMETVS ESGTHEGLFS HQTWEQISSD LTRFQDSMVN
121SFQFSKQDDM PCQVDAGLSI IHVRQKPSEG RTCKKSFSDV SVLDLHQQLQ SREKSHTCDE
181CGKSFCYSSA LRIHQRVHMG EKLYNCDVCG KEFNQSSHLQ IHQRIHTGEK PFKCEQCGKG
241FSRRSGLYVH RKLHTGVKPH ICEKCGKAFI HDSQLQEHQR IHTGEKPFKC DICCKSFRSR
301ANLNRHSMVH MREKPFRCDT CGKSFGLKSA LNSHRMVHTG EKRYKCEECG KRFIYRQDLY
361KHQIDHTGEK PYNCKECGKS FRWASGLSRH VRVHSGETTF KCEECGKGFY TNSQRYSHQR
421AHSGEKPYRC EECGKGYKRR LDLDFHQRVH RGEKPYNCKE CGKSFGWASC LLNHQRIHSG
481EKPFKCEECG KRFTQNSQLY THRRVHSGEK PFKCEECGKR FTQNSQLYSH RRVHTGVKPY
541KCEECGKGFN SKFNLDMHQR VHTGERPYNC KECGKSFSRA SSILNHKRLH GDEKPFKCEE
601CGKRFTENSQ LHSHQRVHTG EKPYKCEKCG KSFRWASTHL THQRLHSREK LLQCEDCGKS
661IVHSSCLKDQ QRDQSGEKTS KCEDCGKRYK RRLNLDTLLS LFLNDT
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

706 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.