LSBio
ZNF221 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ZNF221 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all ZNF221 antibodies →
$725.00each
Catalog Number: LS-G114327-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ZNF221 (NM_013359), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Zinc finger protein 221 | ZNF221
Origin Species
Human
NCBI GenBank
NM_013359
SwissProt
Q9UK13

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
71 kDa
Protein Type
Lysate
Amino Acid Sequence
1MISPSLELLH SGLCKFPEVE GKMTTFKEAV TFKDVAVVFT EEELGLLDPA QRKLYRDVML
61ENFRNLLSVG NQPFHQDTFH FLGKEKFWKM KTTSQREGNS GGKIQIEMET VPEAGPHEEW
121SCQQIWEQIA SDLTRSQNSI RNSSQFFKEG DVPCQIEARL SISHVQQKPY RCNECKQSIS
181DVSVFDLHQQ SHSGEKSHTC GECGKSFCYS PALHIHQRVH MGEKCYKCDV CGKEFNQSSH
241LQTHQRVHTG EKPFKRGQCG KGFHSRSALN VHCKLHTGEK PYNCEECGKA FIHDSQLQEH
301QRIHTGEKPF KCDICGKSFR VRSRLNRHSM VHTGEKPFRC DTCGKNFRQR SALNSHSMVH
361IEEKPYKCEQ CGKGFICRRD FCKHQMVHTG EKPYNCKECG KTFRWSSCLL NHQQVHSGQK
421SFKCEECGKG FYTNSRRSSH QRSHNGEKPY NCEECGKDYK RRLDLEFHQR VHTGERPYNC
481KECGKSFGWA SCLLKHQRLH SGEKPFKCEE CGKRFTQSTQ LHSHQTCHTG EKLYKCEQCE
541KGYNSKFNLD MHQRVHRGER PYNCKECGKS FGWASCLLKH QRLHSGEKPL KSGVWEEIYS
601EFTASFTSVS LCGRKAI
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

617 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.